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KMID : 0363420130270040017
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Journal of Korean Oriental Pediatrics
2013 Volume.27 No. 4 p.17 ~ p.30
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The Study on Anti-obesity Effects of Mulberry Leaves Contained Herbal Mixture
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Park Jong-Ik
Kang Kyung-Ha
Park Eun-Jung
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Abstract
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Objectives: This experimental study was designed to investigate the effects of Mulberry leaves contained herbal mixture (MLHM) on body weight, serum lipid level and adipocyte differentiation in high fat diet-fed obese mice.
Methods: Four-week old mice (wild-type C57/BL6) were used for all experiments. Cells were incubated with MLHM at the indicated concentration (0.04-4mg/㎖) for 24h, and growth rate was assessed by MTT ((3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide) assay. 3T3-L1 preadipocytes were incubated in DMEM for 2 days with the indicated concentrations of MLHM, and on Day 6, the cells were fixed and the cellular lipid contents were assessed by Oil-Red-O staining. The expression of peroxisome proliferator-activated receptor γ (PPAR γ) and cytidine-cytidine-adenosine-adenosinethymine (CCAAT)/enhancer-binding proteins α (C/EBP α) as adipocyte-specific proteins were determined by real time RT-PCR and western blotting. In addition, body weight gain and serum lipid levels were measured in the mice with obesity induced by the high fat-diet for four weeks.
Results: Though MLHM did not show toxicity even at the concentration of 4㎎/ml, MLHM significantly inhibited the
differentiation of 3T3-L1 preadipocites in a dose-dependent manner. Also, MLHM significantly reduced the expressions of PPAR γ and C/EBP α in a dose-dependent manner. Furthermore, MLHM significantly reduced body weight gain and LDL-cholesterol contents in high fat diet-fed obese mice.
Conclusions: These results demonstrate that MLHM exerts anti-obesity effect in 3T3-L1 cells and mice with obesity by high-fat diet.
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KEYWORD
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Mulberry leaves, Obesity, Serum lipid, 3T3-L1 cell, Adipocyte differentiation, PPAR γ, C/EBP α
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